MicroRNA-155 contributes to preeclampsia by down-regulating CYR61.

نویسندگان

  • Yanqing Zhang
  • Zhenyu Diao
  • Li Su
  • Haixiang Sun
  • Ruotian Li
  • Hengmi Cui
  • Yali Hu
چکیده

OBJECTIVE The aim of this study was to characterize the molecular mechanism of preeclampsia (PE) development through miR-155. STUDY DESIGN PE and normal placentas were used to measure miR-155 and cysteine-rich protein 61 (CYR61) expression. CYR61 3' untranslated region was validated as the target of miR-155 using in vitro transfections. miR-155 and CYR61 expression levels were assessed by real-time reverse transcription polymerase chain reaction or Western blot. RESULTS An inverse correlation was found between miR-155 and CYR61 expression levels, with miR-155 up-regulated and CYR61 down-regulated in PE tissues. Luciferase assays and CYR61 transfection assays experimentally validated that miR-155 efficiently targets the 3' untranslated region of CYR61. CONCLUSION This study reported for the first time that overexpression of miR-155 contributes to PE development by targeting and down-regulating angiogenic regulating factor CYR61, leading to pathological alterations. This finding not only characterizes a new mechanism for the disease but also provides a potential therapeutic target.

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عنوان ژورنال:
  • American journal of obstetrics and gynecology

دوره 202 5  شماره 

صفحات  -

تاریخ انتشار 2010